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资源与环境微生物技术

王禄山

教授
博士/硕士生导师
0531-88366202
个人简介 科研项目 学术论文 其他介绍

受教育经历

1999/09 – 2004/06,bet365 코리아,生命科学学院,博士

1992/09 – 1996/06,bet365 코리아,微生物学系,学士


工作经历

2014/01 – 至 今,禹城市人民政府挂职副市长

2013/09 – 至 今,bet365 코리아,生命科学学院,博士生导师

2011/12 – 至 今,bet365 코리아,生命科学学院,教授

2006/09 – 2011/12,bet365 코리아,生命科学学院,副教授

2004/07 – 2006/09,bet365 코리아,生命科学学院,讲师

1996/07 – 1999/09,济南科贝尔生物工程有限公司,助理工程师


科研方向

微生物生理生化及生物信息学

1.碳水化合物活性酶分子的理性设计

2.秸秆资源的生物转化与资源化利用

3.畜禽废弃物生物降解与资源转化利用

研究成果

主持6项国家自然科学基金面上项目、1项国家重点研发计划子课题、1项973项目子课题、1项科技部重大专项国家项目及多省部项目的资助,累计科研经费近700余万元。在Mole Biol Evol, Biotechnol Bioeng,Electrophoresis, Biosource Technol, J Phys Chem B,Phys. Chem. Chem. Phys.等已发表SCI论文30余篇,发表论文后引起国内外同行的较大关注。已经出版著作(译著)4部,累计180余万字。

代表论文

1) Xukai Jiang, Guanjun Chen, Lushan Wang*. Structural and dynamic evolution of the amphipathic N-terminus diversifies enzyme thermostability in the glycoside hydrolase family 12. Phys. Chem. Chem. Phys., 18: 21340-21350, 2016

2) Weili Gong, Huaiqiang Zhang, Li Tian, Shijia Liu, Xiuyun Wu, Fuli Li, Lushan Wang*. Determination of the modes of action and synergies of xylanases by analysis of xylooligosaccharide profiles over time using fluorescence-assisted carbohydrate electrophoresis, Electrophoresis, 37: 1640–1650, 2016

3) Li Tian, Shijia Liu, Shuai Wang, Lushan Wang*. Ligand-binding specificity and promiscuity of the main lignocellulolytic enzyme families as revealed by active-site architecture analysis Scientific Reports, 6:23605, 2016.

4) Lili Zhang, Huaiqiang Zhang, Zhiheng Wang, Guanjun Chen, Lushan Wang*. Dynamic changes of the dominant functioning microbial community in the compost of a 90-m3 aerobic solid state fermentor revealed by integrated meta-omics. Bioresource Technology, 203: 1–10, 2016.

5) Xiaomei Zhang, Shuai Wang, Xiuyun Wu, Shijia Liu, Dandan Li, Hao Xu, Peiji Gao, Guanjun Chen, Lushan Wang*. Subsite-specific contributions of different aromatic residues in the active site architecture of glycoside hydrolase family 12. Scientific Reports 5: 18357 2015.

6) Shijia Liu, Shangjin Shao, Linlin Li, Zhi Cheng, Li Tian, Peiji Gao, Lushan Wang*. Substrate-binding specificity of chitinase and chitosanase as revealed by active-site architecture analysis. Carbohydrate Research, 418(12):50-56, 2015

7) Lili Zhang, Haixia Ma, Huaiqiang Zhang, Luying Xun, Guanjun Chen, Lushan Wang*. Thermomyces lanuginosus is The Dominant Fungus in Maize Straw Composts. Bioresource Technology, 197:266–275,2015

8) Weili Gong, Huaiqiang Zhang, Shijia Liu, Lili Zhang, Peiji Gao, Guanjun Chen, Lushan Wang*. Comparative Secretome Analysis of Aspergillus niger, Trichoderma reesei, and Penicillium oxalicum During Solid-State Fermentation. Applied Biochemistry and Biotechnology, 177(6), 1252-1271, 2015

9) Qing Zhang, Xiaomei Zhang, Peipei Wang, Dandan Li, Guanjun Chen, Peiji Gao, Lushan Wang*. Determination of the action modes of cellulases from hydrolytic profiles over a time course using fluorescence-assisted carbohydrate electrophoresis, Electrophoresis, 36: 910–917,2015

10) Xiaomei Zhang, Ning Liu, Fan Yang, Jinghua Li, Lushan Wang*, Guanjun Chen and Peiji Gao. In Situ Demonstration and Quantitative Analysis of the Intrinsic Properties of Glycoside Hydrolases. Electrophoresis, 33(2):280-287, 2012

11) Jinghua Li,Lushan Wang*. Why Substituting The Asparagine At Position 35 In Bacillus Circulans Xylanase With An Aspartic Acid Remarkably Improves The Enzymatic Catalytic Activity? A Quantum Chemistry-Based Calculation Study. Polymer Degradation and Stability , 96:1009-1014, 2011.

12) Zheng Zhang, Jie Huang, Zengfang Wang, Lushan Wang* and Peiji Gao. Impact of indels on the flanking regions in structural domains. Molecular biology and evolution, 28(1):291–301,2011.

13) Jinghua Li, Likai Du, Lushan Wang*. Glycosidic-Bond Hydrolysis Mechanism Catalyzed by Cellulase Cel7A from Trichoderma reesei: A Comprehensive Theoretical Study by Performing MD, QM, and QM/MM Calculations. The Journal of Physical Chemistry B, 114:15261–15268, 2010.

14) Lu-shan Wang, Yu-zhong Zhang, Pei-ji Gao*. Dongxia Shi, Hongwen Liu, Hongjun Gao, Changes in the structural properties and rate of hydrolysis of cotton fibres during extended enzymatic hydrolysis. Biotechnol. Bioeng. 93: 443-456, 2006.


著作/译著:

1). 王禄山,耿存亮,段晓云 等译 生物燃料的计算模拟,化学工业出版社. 40.4万字, 2012

2). 王禄山,张正 等译,纤维素降解的超分子机器——纤维小体,化学工业出版社. 40.2万字,2011

3). 王禄山,张正 等译,生物质抗降解屏障——解构植物细胞壁产生物能,化学工业出版社. 65.4万字,2010

4). 王禄山,高培基,生物信息学应用技术,化学工业出版社. 37.5万字,2008。

奖励:

1) 王禄山;2013年度bet365 코리아课堂教学质量优秀教师;bet365 코리아,2014-04

2)陈冠军,张可炜,季明杰,王禄山。山东省精品课程立项:生物化学(2012BK178),山东省教育厅, 2012

3)王禄山。糖苷水解酶酸碱催化机制的理论探索,山东省人民政府学位委员会,山东省优秀学士学位论文指导教师,2012

4)饶子和,王禄山。鸟类支气管炎病毒IBV非结构蛋白nsp2的表达纯化、结晶、结构与功能初探,山东省人民政府学位委员会,山东省优秀学士学位论文指导教师,2010

5)王禄山,高培基。纤维素酶CBHI连续催化机制的分子模拟研究,山东省人民政府学位委员会,山东省优秀学士学位论文指导教师,2009

6) 李越中,王禄山,张治国,高培基,黎志凤。生物信息学课程体系的建设,bet365 코리아,校级教学成果二等奖, 2008


专利:

1)王禄山,陈冠军,高培基,李亭亭。一种微米级均一纤维素粉末及其制备方法与应用, 2011.5.4,中国,ZL 2007100114797.8。

5.学术兼职

1)中国中国生物化学与分子生物学会第五届酶学专业委员会委员,2015-9-19到2019-9-18

2)中国生物发酵产业协会第二届工程技术委员会委员

(第二届全国发酵工程技术工作委员会委员)

3)德州市生物(功能糖)产业技术创新战略联盟技术顾问